Composite

Part:BBa_K3386004:Design

Designed by: Daria Pająk, Gustav Aberg   Group: iGEM20_Aalto-Helsinki   (2020-10-22)


Biosensor device for erythromycin detection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 542
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 436
    Illegal XhoI site found at 87
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2353


Design Notes

No design considerations.


Source

View the source of each basic part.

References

1. mphR - Macrolide 2'-phosphotransferase I repressor A, erythromycin resistance repressor protein - Escherichia coli - mphR gene & protein. (2020). Retrieved 7 October 2020, from https://www.uniprot.org/uniprot/A0A023UEV3

2. Noguchi, N., Takada, K., Katayama, J., Emura, A., & Sasatsu, M. (2000). Regulation of Transcription of themph(A) Gene for Macrolide 2′-Phosphotransferase I inEscherichia coli: Characterization of the Regulatory Gene mphR(A). Journal Of Bacteriology, 182(18), 5052-5058. doi: 10.1128/jb.182.18.5052-5058.2000

3. UniProt: a worldwide hub of protein knowledge. (2018). Nucleic Acids Research, 47(D1), D506-D515. doi: 10.1093/nar/gky1049